16.10.2017

Target and identify: triazene linker helps identify azidation sites of labelled proteins

A Triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy. A method for identifying probe modification of proteins via tandem mass spectrometry was developed.


A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

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